bodipy 493 503  (MedChemExpress)

Journal: Materials Today Bio

Article Title: Bioinspired lipid droplets nanoplatform for periodontitis therapy: Integrated antibacterial, mitochondrial repair, and immunomodulatory functions

doi: 10.1016/j.mtbio.2026.102808

Figure Lengend Snippet: Bioengineering and characterization of GA@LDs-CRAMP. A) Schematic of GA passive loading into RAW264.7 macrophage-derived LDs. B) Cell viability of RAW264.7 macrophages treated with GA at varying concentrations (n = 5). C) Confocal images showing time-dependent intracellular distribution of Cy5.5-labeled GA (red) with LDs stained by BODIPY 493/503 (green). Scale bar: 10 μm. D) Fluorescence imaging of LPS concentration/duration-dependent LD biogenesis (BODIPY 493/503). Scale bar: 10 μm. E) Number (a) , size (b) of LDs in RAW264.7 cells under stimulation with different LPS concentrations (n = 10), and qRT-PCR quantification of PLIN2 mRNA expression level (c) (n = 3). F) Number (a) , size (b) of LDs in RAW264.7 cells under LPS stimulation for different durations (n = 10), and qRT-PCR quantification of PLIN2 mRNA expression level (c) (n = 3). G) Confocal immunofluorescence images of CRAMP (red) and LDs (green) in 1 μg mL −1 LPS-stimulated RAW264.7 cells. Scale bar: 5 μm. H) Western blot verification of the expression of PLIN2 and CRAMP on the surface of GA@LDs-CRAMP isolated from samples with different LPS concentrations (a) , CRAMP expression is LPS concentration-dependent (b) and shows a good linear relationship (c) . I) Western blot verification of the expression of PLIN2 and CRAMP on the surface of GA@LDs-CRAMP isolated from samples with different numbers of LDs (a) , CRAMP expression is linearly dependent on the number of LDs (b) and consistent with the expression level of PLIN2 (c) . J) Workflow diagram for GA@LDs-CRAMP synthesis via sequential GA encapsulation and LPS-mediated CRAMP recruitment. K) Optical images (a) Layered suspension post-centrifugation, (b) lyophilized powder, (c) aqueous dispersion. L) : TEM micrographs of (a) LDs-CRAMP and (b) GA@LDs-CRAMP. Scale bar: 100 nm. M) Hydrodynamic diameter distribution by DLS: (a) LDs-CRAMP, (b) GA@LDs-CRAMP, and (c) zeta potential measurements (n = 3). N) UV–Vis absorbance spectra of LDs-CRAMP, GA and GA@LDs-CRAMP. O) pH-responsive GA release profiles (pH 5.5 PBS, 72 h). P) Western blot bands of CRAMP expression on the surface of GA@LDs-CRAMP stored in vitro for 12 h, 24 h, 48 h, and 72 h (n = 3). Q) Quantitative analysis of the stability of CRAMP expression on the surface of GA@LDs-CRAMP stored in vitro for 72 h (n = 3). R) Changes in the number and size of intracellular LDs during the synthesis process (n = 20). S) Stability of GA@LDs-CRAMP in PBS and 10 % FBS-DMEM over 72 h (n = 5). ns>0.05, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Synthesis conditions were screened by staining LDs with BODIPY 493/503 (5 μM, MedChemExpress, USA) and counterstaining nuclei with DAPI (7 μM, Beyotime, China).

Techniques: Derivative Assay, Labeling, Staining, Fluorescence, Imaging, Concentration Assay, Quantitative RT-PCR, Expressing, Immunofluorescence, Western Blot, Isolation, Encapsulation, Suspension, Centrifugation, Dispersion, Zeta Potential Analyzer, In Vitro